Papyrus 53, signed by 𝔓53, is an early copy of the New Testament in Koine Greek. It is a papyrus manuscript containing parts of the Gospel of Matthew and the Acts of the Apostles: it contains only Matthew 26:29-40 and Acts 9:33-10:1. The manuscript palaeographically had been assigned to the 3rd century. These two fragments were found together, they were part of a codex containing the four Gospels and Acts or Matthew and Acts.